Prevalence of avian infectious bronchitis virus in broiler chicken farms in south of Iraq, 2014 – 2015

Authors

  • Masoud Hashemzadeh Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • Mehdi Vasfi Marandi Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Omid Madadgar Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Vahid Karimi Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Waleed Seger Department of Pathology and Poultry Diseases, Faculty of Veterinary Medicine, University of Basra, Basra, Iraq | Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:

Avian infectious bronchitis (IB), caused by a gammacoronavirus, is an OIE-listed (List B) disease and characterized by respiratory and renal involvements, causing high mortality, and economic loss in both layers and broilers. In comparison with other diagnostic methods, real-time polymerase chain reaction (RT-PCR) and conventional RT-PCR are potent, more sensitive and faster techniques for infectious bronchitis virus (IBV) detection. This research was conducted to detect IBV using specific primers of IB in three governorates (Basra, Thi-Qar and Muthana) in the south of Iraq. Tracheal specimens were collected from 46 IB suspected commercial broiler flocks. XCE2+ and XCE2- Primers, which amplify all IBV serotypes, were used. Primers MCE1+, BCE1+ and DCE1+ were used to amplify the specific nucleotide sequences of Massachusetts, 793/B and D274 genotypes, respectively. The results of real-time RT-PCR of this study showed that 34 (74.00%) out of 46 infected flocks were positive to IBV. The results of nested PCR showed that 50.00% and 5.89% of positive samples were belonged to genotypes 793/B and Massachusetts, respectively, and the remaining positive (44.11%) were unknown. The results indicate presence of  Massachusetts and 793/B IBV strains in commercial broilers in southern Iraq.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

prevalence of avian infectious bronchitis virus in broiler chicken farms in south of iraq, 2014 – 2015

avian infectious bronchitis (ib), caused by a gammacoronavirus, is an oie-listed (list b) disease and characterized by respiratory and renal involvements, causing high mortality, and economic loss in both layers and broilers. in comparison with other diagnostic methods, real-time polymerase chain reaction (rt-pcr) and conventional rt-pcr are potent, more sensitive and faster techniques for infe...

full text

Genotyping of Infectious bronchitis viruses isolated from broiler chicken farms in Iran during 2015-2016

BACKGROUND: Avian infectious bronchitis is considered as an important viral disease worldwide. Genotyping based on the S1 subunit of spike protein gene of the causative agent, avian infectious bronchitis virus, can be used to classify IBV isolates. Objective: This survey was carried out to characterize the infectious bronchitis virus (IBV) genotypes circulating in Iran and determine their preva...

full text

Emergence of variant avian infectious bronchitis virus in India

Background: Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multipl...

full text

Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015

The aim of this study was to provide information on the molecular characteristic and the phylogenic relationship of infectious bronchitis viruses (IBV) strains in Bushehr province in comparison to other strains reported in the Middle East. Samples were collected from broiler flocks in Bushehr province during 2014 - 2015. These flocks had respiratory problems such as gasping, sneezing and bronch...

full text

Full-length Characterization of S1 Gene of Iranian QX Avian Infectious Bronchitis Virus Isolates, 2015

Background and Aims: Avian infectious bronchitis (IB) has prevalent in the most chicken farms during recent years, in spite of the IB vaccination program which has been widely performed in Iran. To better understand the molecular epidemiology of IBV in Iran, the full length sequences of S1 gene of Iranian QX IBVs were determined and phylogenetic analysis was done using some sequences of IBV. M...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 4

pages  317- 321

publication date 2016-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023